percolltm (Cytiva Europe)
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99
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Cytiva Europe
percolltm
Percolltm, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 99/100, based on 13081 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/percolltm/Percoll/us12655190-241-18-19
Average 99 stars, based on 13081 article reviews
Percolltm, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 99/100, based on 13081 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/percolltm/Percoll/us12655190-241-18-19
Average 99 stars, based on 13081 article reviews
percolltm - by Bioz Stars,
2026-11
99/100 stars
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other:Article Title: NK cell compositions and preparations for immunotherapy and methods for their production Article Snippet: Other separation methods that can be used include the use of commercially available products FICOLL or FICOLL-PAQUE or Article Title: Carnosine as a protective metabolic mediator in inflammatory lung injury by inhibiting macrophage infiltration and M1-like polarization Article Snippet: The following reagents were also used in our study: 1640 (Gibco, Grand Island, United States), FBS (Gibco, Grand Island, United States), trypsin (Amresco, United States), EDTA (Amresco, United States), ELISAKit for TNF-α and IL-6 (R&DSystems, Minneapolis, United States), Annexin V-FITC/PI Kit (BD, San Jose, United States), APC-conjugated anti-mouse CD8 (BioLegend, San Diego, United States), BV421-conjugated anti-mouse CD86 (BioLegend, San Diego, United States), BV711-conjugated anti-mouse CD64 (BioLegend, SanDiego, United States), FITC anti-mouseMERTK Monoclonal Antibody (BioLegend, San Diego, United States), PECY7conjugated anti-mouse CD11b (BioLegend, San Diego, United States), PE-conjugated anti-mouse CD3 (BioLegend, San Diego, United States), PEconjugated anti-mouse B220 (BioLegend, San Diego, United States), AlexaFluor® 594 anti-mouse CD326 (Ep-CAM) Antibody (BioLegend, San Diego, United States), Alexa Fluor® 647 mouse anti-Siglec-F (BioLegend, San Diego, United States), eFluor 570 mouse anti-F4/80 (BioLegend, San Diego, United States), eBioscienceTM Fixable Viability Dye eFluorTM 780 (eBioscienceTM, San Diego, United States), DNase I (Roche, Basel, Switzerland), DTT (Merck–Roche, Basel, Switzerland), collagenase IV (Sigma-Aldrich, St. Louis, United States), PrecisionCount BeadsTM (BioLegend, San Diego, United States), Fixation/ Permeabilization Concentrate (Invitrogen, Carlsbad, United States), Article Title: Analysis of GRK2 aggregation in the pathology of Alzheimer disease in animal models Article Snippet: Percoll , Incubation:Article Title: ALS-linked mutant TDP-43 in oligodendrocytes induces oligodendrocyte damage and exacerbates motor dysfunction in mice Article Snippet: The C Tubes were placed on a gentleMACSTM dissociator (Miltenyi Biotec), and the tissues were dissociated into single-cell suspensions for 30 min at 37oC. .. The cell suspensions were passed through 70-μm cell strainers (pluriSelect Life Science, Leipzig, Germany) with 0.5% (w/v) BSA (Nacalai Tesque) in PBS using the plungers of 5-mL syringes and centrifuged at 600 × g , and room temperature for 5 min. After aspirating the supernatant, the cell suspensions were mixed with 40% ( Article Title: ALS-linked mutant TDP-43 in oligodendrocytes induces oligodendrocyte damage and exacerbates motor dysfunction in mice. Article Snippet: The C Tubes were placed on a gentleMACSTM dissociator (Miltenyi Biotec), and the tissues were dissociated into single-cell suspensions for 30 min at 37oC. .. The cell suspensions were passed through 70-μm cell strainers (pluriSelect Life Science, Leipzig, Germany) with 0.5% (w/v) BSA (Nacalai Tesque) in PBS using the plungers of 5-mL syringes and centrifuged at 600 × g, and room temperature for 5 min. After aspirating the supernatant, the cell suspensions were mixed with 40% ( Blocking Assay:Article Title: ALS-linked mutant TDP-43 in oligodendrocytes induces oligodendrocyte damage and exacerbates motor dysfunction in mice Article Snippet: The C Tubes were placed on a gentleMACSTM dissociator (Miltenyi Biotec), and the tissues were dissociated into single-cell suspensions for 30 min at 37oC. .. The cell suspensions were passed through 70-μm cell strainers (pluriSelect Life Science, Leipzig, Germany) with 0.5% (w/v) BSA (Nacalai Tesque) in PBS using the plungers of 5-mL syringes and centrifuged at 600 × g , and room temperature for 5 min. After aspirating the supernatant, the cell suspensions were mixed with 40% ( Article Title: ALS-linked mutant TDP-43 in oligodendrocytes induces oligodendrocyte damage and exacerbates motor dysfunction in mice. Article Snippet: The C Tubes were placed on a gentleMACSTM dissociator (Miltenyi Biotec), and the tissues were dissociated into single-cell suspensions for 30 min at 37oC. .. The cell suspensions were passed through 70-μm cell strainers (pluriSelect Life Science, Leipzig, Germany) with 0.5% (w/v) BSA (Nacalai Tesque) in PBS using the plungers of 5-mL syringes and centrifuged at 600 × g, and room temperature for 5 min. After aspirating the supernatant, the cell suspensions were mixed with 40% ( Labeling:Article Title: Devices and disposables for patient-specific cell therapy manufacturing Article Snippet: .. Density based separation processes performed with the sample processing unit of the inventive system including, without limitation, cell washing, buffy coat generation, density based separation using density media, e.g. FicollTM, Sedimentation:Article Title: Devices and disposables for patient-specific cell therapy manufacturing Article Snippet: .. Density based separation processes performed with the sample processing unit of the inventive system including, without limitation, cell washing, buffy coat generation, density based separation using density media, e.g. FicollTM, Article Title: Fish Erythrocyte Extracellular Traps (FEETs) are an evolutionarily conserved cellular process triggered by different stimuli. Article Snippet: Fish erythrocytes remain nucleated, unlike mammalian erythrocytes that undergo enucleation during maturation.. Besides oxygen transport, fish erythrocytes are capable of several immune defence processes and thus these cells are candidates for carrying out ETotic responses.. ETosis is an evolutionarily conserved innate immune defence process found in both vertebrates and invertebrates, which involves the extrusion of DNA studded with antimicrobial effector proteins into the extracellular space that traps and kills microorganisms. Isolation:Article Title: Fish Erythrocyte Extracellular Traps (FEETs) are an evolutionarily conserved cellular process triggered by different stimuli. Article Snippet: Fish erythrocytes remain nucleated, unlike mammalian erythrocytes that undergo enucleation during maturation.. Besides oxygen transport, fish erythrocytes are capable of several immune defence processes and thus these cells are candidates for carrying out ETotic responses.. ETosis is an evolutionarily conserved innate immune defence process found in both vertebrates and invertebrates, which involves the extrusion of DNA studded with antimicrobial effector proteins into the extracellular space that traps and kills microorganisms. |